Mastering the Gram-negative bacterial barrier – Chemical approaches to increase bacterial bioavailability of antibiotics

To win the battle against resistant, pathogenic bacteria, novel classes of anti-infectives and targets are urgently needed. Bacterial uptake, distribution, metabolic efflux pathways antibiotics in Gram-negative bacteria determine what we here refer to as bacterial bioavailability. Understanding these mechanisms from a chemical perspective is essential for anti-infective activity hence, drug discovery well delivery. A systematic critical discussion bacterio, vitro silico assays reveals that sufficiently accurate holistic approach still missing. We expect new findings based on bioavailability guide future research.